Analysis Note

ControlPOSITIVE CONTROL: Conditioned, serum-free medium from (PMA-treated) human fibrosarcoma HT-1080 cells. Bladder, breast, ovarian carcinomas.

Application

Immunohistochemistry (frozen and formalin-fixed/paraffin*): 2-4 μg/mL for 60 minutes at room temperature.

* Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperature for 20 minutes.

Immunoprecipation: use protein G (2 μg/mg protein lysate)

Optimal working dilutions must be determined by end user.

Research CategoryCell Structure

Research Sub CategoryMMPs & TIMPs

This Anti-MMP-13 Antibody, clone VIIIA2 is validated for use in IH for the detection of MMP-13.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Human recombinant collagenase-3 protein.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Liquid in 10 mM PBS, pH 7.4, with 0.2% BSA and 15 mM sodium azide.

Specificity

It recognizes proteins of ~60 kDa and ~48 kDa which are identified as pro (latent) and activate forms of matrix metalloproteinase-13 (MMP-13; also known as Collagenase-3). Shows no cross reactivity with the pro and active forms of other MMPs. Human collagenase-3 (MMP13) is a recently identified member of the matrix metalloproteinase (MMP) family that is expressed in breast carcinomas and in articular cartilage from arthritic patients. The MMP-13 gene has been isolated and characterized. This gene is composed of 10 exons and 9 introns and spans over 12.5 kb. The overall organization of the collagenase-3 gene is similar to that of other MMP genes clustered at chromosome 11q22, including fibroblast collagenase (MMP-1), matrilysin (MMP-7), and macrophage metalloelastase (MMP-12), but is more distantly related to genes coding for stromelysin-3 (MMP-11), gelatinase-A (MMP-2), and gelatinase-B (MMP-9), which map outside of this gene cluster. Cellular Localization: Cytoplasmic

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 12 months.